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Domain-based structural studies of Replication Protein A: analysis of an RPA32N phospho-mimic mutant and the role of RPA70N in binding ssDNA
(2010-08-03)
Replication Protein A (RPA) is the primary eukaryotic ssDNA binding protein utilized in preventing secondary structure formation and re-annealing of unwound DNA strands, thereby controlling access to DNA templates in diverse ...
Structural separations of endogenous and exogenous compounds directly from tissue sections by ion mobility – mass spectrometry
(2010-08-04)
Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) can provide the spatial distribution, relative abundance, and molecular identity of thousands of analytes directly from tissue sections. Due to the ...
Proteomic Signatures of Epidermal Growth Factor Receptor Signaling
(2012-01-25)
Analysis of cellular signaling networks typically involves targeted
measurements of phosphorylated protein intermediates. However,
phosphoproteomic analyses usually require affinity enrichment of
phosphopeptides and ...
NRIF is an essential component of apoptotic signaling by the p75 neurotrophin receptor
(2005-01-27)
Activation of the p75 neurotrophin receptor leads to a variety of effects within the nervous system, including neuronal apoptosis. Both c-Jun N-terminal kinase (JNK) and the tumor suppressor p53 have been reported to be ...
The DNA Cleavage Reaction of Human Type II Topoisomerases
(2009-02-04)
The work presented in this dissertation examines the DNA cleavage activity of human topoisomerase II. Type II topoisomerases are required for removing DNA knots and tangles and are important chemotherapy drug targets. The ...
RNA Recognition by the Histone Demethylase LSD1 and Proteinaceous RNase P: Characterization of the Binding of Highly Structured RNAs by Enzyme Complexes
(2018-02-13)
This study investigates the recognition of structured RNAs by two essential enzymes, proteinaceous RNase P (PRORP) and lysine-specific demethylase-1 (LSD1). PRORP binds and cleaves human mitochondrial precursor tRNAs in ...
Regulation of replication fork stability by ssDNA binding proteins
(2018-02-26)
The replication stress response (RSR) maintains genome stability and promotes the accurate duplication of the genome. ssDNA binding proteins are integral components of the RSR and have been extensively studied for years. ...
Effects of DNA Geometry and Topoisomerase II Poisons on Human and Bacterial Type II Topoisomerases
(2017-03-15)
The topological state of DNA has a dramatic effect on nucleic acid processes in cells. Type II topoisomerases are necessary enzymes that help regulate the topological state of the genome, but their activity requires the ...
Mechanism of Quinolone Action and Resistance in Bacterial and Human Type II Topoisomerases
(2014-03-17)
Quinolones are the most commonly prescribed antibacterials worldwide; however, their clinical utility is threatened by the rising occurrence of resistance. Quinolones kill bacterial cells by increasing the level of DNA ...
Oxidative metabolism of exocyclic DNA adducts
(2008-03-13)
Multiple exocyclic DNA adducts arise from reactions of lipid and DNA peroxidation products with DNA bases. These endogenous lesions are mutagenic; therefore, monitoring adduct levels may provide a means of assessing genomic ...