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Now showing items 111-119 of 119
High Spatial Resolution Multi-omic Investigations of Staphylococcus aureus Biofilm Layers: Visualizing Daptomycin-Resistance
(2022-09-14)
Staphylococcus aureus infections pose a serious threat to human health with the increasing incidence of microbial resistance to last-line antibiotics such as daptomycin. Many S. aureus infections present as a biofilm wherein ...
RADX protein interactions regulate replication fork stability
(2022-11-02)
Genome integrity requires complete and accurate DNA replication once per cell division cycle. Replication stress poses obstacles to this process that must be overcome to prevent replication fork collapse. An important ...
Replication Fork Uncoupling Causes Nascent Strand Degradation and Fork Reversal
(2022-11-22)
Genotoxic agents can cause nascent strand degradation (NSD) and fork reversal during DNA replication. NSD and fork reversal are crucial biochemical mechanisms that maintain genome stability and they are frequently exploited ...
The role of human SMARCA5 in chromatin structure and function
(2023-01-30)
Genetic models suggested that SMARCA5 was required for DNA templated events including transcription, DNA replication and DNA repair. We engineered a degron tag into the endogenous alleles of SMARCA5, a catalytic component ...
Identifying Gene Regulatory Activity Divergence in Cis and Trans with ATAC-STARR-seq
(2023-03-24)
Gene regulatory divergence between species can result from cis-acting local changes to regulatory element DNA sequences or global trans-acting changes to the regulatory environment. Understanding how these mechanisms drive ...
Basis for the Discrimination of Supercoil Handedness During DNA Cleavage by Human and Bacterial Type II Topoisomerases
(2023-03-14)
Type II topoisomerases are essential enzymes that regulate the topological state of DNA. These enzymes function by performing a double-stranded DNA passage reaction, the process of which generates a covalent enzyme-cleaved ...
ARRESTIN-MEDIATED EFFECTOR ACTIVATION
(2023-07-18)
Nonvisual arrestins (arrestin-2 and arrestin-3) regulate a wide range of signaling events, most notably when bound to active G protein-coupled receptors (GPCRs). Among the known effectors recruited by GPCR-bound arrestins ...
ARRESTIN-MEDIATED EFFECTOR ACTIVATION
(2023-07-18)
Nonvisual arrestins (arrestin-2 and arrestin-3) regulate a wide range of signaling events, most notably when bound to active G protein-coupled receptors (GPCRs). Among the known effectors recruited by GPCR-bound arrestins ...
STRUCTURAL BASIS OF O-SIALOGLYCAN RECOGNITION AND SELECTIVITY IN α2,3 SELECTIVE STREPTOCOCCAL SERINE RICH REPEAT ADHESINS AND α2,6 LINKAGE SPECIFIC BACTERIAL SIALYLTRANSFERASES
(2023-10-30)
Glycans play an essential role in biological processes, but our knowledge of their structure and function is limited as compared to other biological molecules. Glycan structure is highly variable. In vivo, glycan structure ...