High-Throughput Epitope Determination of HIV-1 Polyclonal B Cell Repertoires
dc.contributor.advisor | Georgiev, Ivelin | |
dc.creator | Venkat, Rohit | |
dc.date.accessioned | 2020-12-29T15:31:16Z | |
dc.date.created | 2020-12 | |
dc.date.issued | 2020-11-18 | |
dc.date.submitted | December 2020 | |
dc.identifier.uri | http://hdl.handle.net/1803/16408 | |
dc.description.abstract | For over 30 years, an HIV-1 vaccine has remained elusive due to the high mutability and tremendous levels of genetic diversity exhibited by the virus. Current research efforts have focused on understanding B cell-encoded antibody responses to HIV-1 infection to help inform vaccine design. Specifically, the analysis of B cell receptor (BCR) specificities arising from infection is valuable for optimizing the design of epitope- and germline-targeting HIV-1 immunogens. Unfortunately, the BCR specificities in an individual are polyclonal and difficult to characterize using currently available methods. Here, we introduce a LIBRA-seq based assay for antibody epitope determination, providing the ability to simultaneously obtain functional information about antibody epitope specificity together with paired heavy- and light-chain B cell receptor sequence for thousands of individual B cells in a polyclonal sample. | |
dc.format.mimetype | application/pdf | |
dc.language.iso | en | |
dc.subject | HIV-1 | |
dc.subject | vaccine design | |
dc.subject | antibody | |
dc.subject | B cell receptor | |
dc.subject | epitope specificity | |
dc.subject | LIBRA-seq | |
dc.subject | high-throughput | |
dc.title | High-Throughput Epitope Determination of HIV-1 Polyclonal B Cell Repertoires | |
dc.type | Thesis | |
dc.date.updated | 2020-12-29T15:31:16Z | |
dc.type.material | text | |
thesis.degree.name | MS | |
thesis.degree.level | Masters | |
thesis.degree.discipline | Chemical & Physical Biology | |
thesis.degree.grantor | Vanderbilt University Graduate School | |
local.embargo.terms | 2022-12-01 | |
local.embargo.lift | 2022-12-01 | |
dc.creator.orcid | 0000-0002-8029-9350 |
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Electronic Theses and Dissertations
Electronic theses and dissertations of masters and doctoral students submitted to the Graduate School.