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Examination of a mouse model lacking the engulfment receptor Jedi

dc.contributor.advisorHiebert, Scott W.
dc.contributor.advisorCarter, Bruce D.
dc.creatorTrevisan, Alexandra J.
dc.date.accessioned2020-09-22T22:13:46Z
dc.date.created2020-05
dc.date.issued2020-03-30
dc.date.submittedMay 2020
dc.identifier.urihttp://hdl.handle.net/1803/16044
dc.description.abstractThe dorsal root ganglia (DRG) house the primary afferent neurons responsible for somatosensation, including pain. We previously identified Jedi-1 (PEAR1/MEGF12) as a phagocytic receptor expressed by satellite glia in the DRG involved in clearing apoptotic neurons during development. Here, we further investigated the function of this receptor in vivo using Jedi-1 null mice. In addition to satellite glia, we found Jedi-1 expression in perineurial glia and endothelial cells, but not in sensory neurons. We did not detect any morphological or functional changes in the glial cells or vasculature of Jedi-1 knockout mice. Surprisingly, we did observe changes in DRG neuron activity. In neurons from Jedi-1 knockout (KO) mice, there was an increase in the fraction of capsaicin-sensitive cells relative to wild type (WT) controls. Patch-clamp electrophysiology revealed an increase in excitability, with a shift from phasic to tonic action potential firing patterns in KO neurons. We also found alterations in the properties of voltage-gated sodium channel currents in Jedi-1 null neurons. These results provide new insight into the expression pattern of Jedi-1 in the peripheral nervous system and indicate that loss of Jedi-1 alters DRG neuron activity indirectly through an intercellular interaction between non-neuronal cells and sensory neurons.
dc.format.mimetypeapplication/pdf
dc.language.isoen
dc.subjectSomatosensation, Jedi, Pain, Pruritus, Phagocytosis, Dorsal root ganglia
dc.titleExamination of a mouse model lacking the engulfment receptor Jedi
dc.typeThesis
dc.date.updated2020-09-22T22:13:46Z
dc.type.materialtext
thesis.degree.namePhD
thesis.degree.levelDoctoral
thesis.degree.disciplineBiochemistry
thesis.degree.grantorVanderbilt University Graduate School
local.embargo.terms2022-05-01
local.embargo.lift2022-05-01
dc.creator.orcid0000-0002-8887-4444


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