dc.creator | Burse, Mallori Jacole | |
dc.date.accessioned | 2020-08-23T16:11:26Z | |
dc.date.available | 2018-06-03 | |
dc.date.issued | 2017-12-05 | |
dc.identifier.uri | https://etd.library.vanderbilt.edu/etd-12042017-090452 | |
dc.identifier.uri | http://hdl.handle.net/1803/15118 | |
dc.description.abstract | The host protein cyclophilin A (CypA) can both stimulate and inhibit HIV-1 infection through its interaction with the viral capsid (CA). CypA enhances the early stages of HIV-1 infection in part by promoting nuclear import of the virus; while the details of its ability to inhibit HIV-1 infection are less clear. This thesis advances our understanding of the mechanisms underlying the ability of CypA to inhibit HIV-1 infection. I demonstrate that CypA inhibits nuclear import of HIV-1 in the presence of inhibitory capsid-binding host proteins TRIM5α and CPSF6, and that inhibition is not a consequence of increased binding of inhibitory factors to the viral capsid. My work also demonstrates that CypA-dependent inhibition depends in part, on a conserved domain of HIV-1 CA which determines interactions with host nuclear pore proteins. These results suggest a common mechanism underlies the ability of CypA to stimulate HIV-1 infectivity in some cells and to inhibit infection in others. | |
dc.format.mimetype | application/pdf | |
dc.subject | restriction factors | |
dc.subject | virus-host Interactions | |
dc.subject | HIV-1 | |
dc.subject | cyclophilin A | |
dc.subject | CypA | |
dc.title | Inhibitory Control of HIV-1 by Cyclophilin A | |
dc.type | dissertation | |
dc.contributor.committeeMember | Christopher Aiken | |
dc.contributor.committeeMember | David Cortez | |
dc.contributor.committeeMember | Kristen Ogden | |
dc.type.material | text | |
thesis.degree.name | PHD | |
thesis.degree.level | dissertation | |
thesis.degree.discipline | Microbiology and Immunology | |
thesis.degree.grantor | Vanderbilt University | |
local.embargo.terms | 2018-06-03 | |
local.embargo.lift | 2018-06-03 | |
dc.contributor.committeeChair | Earl Ruley | |