dc.creator | Garmendia, Craig Alexander | |
dc.date.accessioned | 2020-08-23T16:10:34Z | |
dc.date.available | 2010-12-15 | |
dc.date.issued | 2008-12-15 | |
dc.identifier.uri | https://etd.library.vanderbilt.edu/etd-12042008-144306 | |
dc.identifier.uri | http://hdl.handle.net/1803/15097 | |
dc.description.abstract | Exposure to endogenous and exogenous chemicals can lead to modification of DNA and potentially lead to cancer. Frameshift mutations may be a consequence of these modifications to DNA. Oligonucleotides were synthesized that contain a site specific a site specific C8-deoxyguanosine adduct 2-amino-3-methylnaphtho[1,2-d]imidazole (CCIQ), which is an analogue of the food mutagen 2-amino-3-methylimidazo[4,5-f]quinoline (IQ), and the adducts R- and S-á-methyl-ã-hydroxy-1,N2-propano-2’-deoxyguaosine and ã-hydroxy-1,N2-propano-2’-deoxyguanosine in frameshift prone sequences. In vitro replication studies were conducted to examine which nucleotide was incorporated opposite the adducts by a variety of DNA polymerases. The sequence and yields of the extension products were determined using tandem mass spectrometry. | |
dc.format.mimetype | application/pdf | |
dc.subject | Oligonucleotides -- Synthesis | |
dc.subject | Tandem Mass Spectrometry | |
dc.subject | Frameshift | |
dc.subject | DNA Adducts | |
dc.subject | Acrolein | |
dc.subject | Crotonaldehyde | |
dc.subject | Chemical carcinogenesis | |
dc.title | In vitro replication studies of oligonucleotides containing site-specific DNA adducts in frameshift-prone sequences | |
dc.type | thesis | |
dc.contributor.committeeMember | F. Peter Guengerich | |
dc.contributor.committeeMember | Ned A. Porter | |
dc.type.material | text | |
thesis.degree.name | MS | |
thesis.degree.level | thesis | |
thesis.degree.discipline | Chemistry | |
thesis.degree.grantor | Vanderbilt University | |
local.embargo.terms | 2010-12-15 | |
local.embargo.lift | 2010-12-15 | |
dc.contributor.committeeChair | Carmelo J. Rizzo | |