dc.creator | Shaffer, Carrie Leigh | |
dc.date.accessioned | 2020-08-23T15:50:46Z | |
dc.date.available | 2013-11-22 | |
dc.date.issued | 2011-11-22 | |
dc.identifier.uri | https://etd.library.vanderbilt.edu/etd-11222011-140044 | |
dc.identifier.uri | http://hdl.handle.net/1803/14725 | |
dc.description.abstract | Colonization of the human stomach by Helicobacter pylori is an important risk factor for development of gastric cancer. The H. pylori cag pathogenicity island (cag PAI) encodes components of a type IV secretion system (T4SS) that translocates the bacterial oncoprotein CagA into gastric epithelial cells, and CagL is a specialized component of the cag T4SS that binds the host receptor α5β1 integrin. Herein, we describe a mass spectrometry-based approach to identify a T4SS subassembly that contains CagL, CagH, and CagI. We demonstrate that these three proteins are required for CagA translocation into host cells and H. pylori-induced IL-8 secretion by gastric epithelial cells; however, these proteins are not homologous to components of T4SSs in other bacterial species. Moreover, we show that these proteins play key roles in biogenesis of T4SS pili at the bacteria-host cell interface. Collectively, these results highlight the important role played by unique constituents of the H. pylori cag T4SS, and illustrate the marked variation that exists among bacterial T4SSs. | |
dc.format.mimetype | application/pdf | |
dc.subject | bacteriology | |
dc.subject | proteomics | |
dc.subject | Helicobacter pylori | |
dc.subject | pathogenicity | |
dc.title | Role of unique Helicobacter pylori proteins in the Cag pathogenicity island-encoded type IV secretion system | |
dc.type | dissertation | |
dc.contributor.committeeMember | Eric Skaar | |
dc.contributor.committeeMember | Seth Bordenstein | |
dc.contributor.committeeMember | Roy Zent | |
dc.contributor.committeeMember | Andrew Link | |
dc.contributor.committeeMember | Timothy Cover | |
dc.type.material | text | |
thesis.degree.name | PHD | |
thesis.degree.level | dissertation | |
thesis.degree.discipline | Microbiology and Immunology | |
thesis.degree.grantor | Vanderbilt University | |
local.embargo.terms | 2013-11-22 | |
local.embargo.lift | 2013-11-22 | |
dc.contributor.committeeChair | D. Borden Lacy | |