dc.creator | Millsap, Amy Danae | |
dc.date.accessioned | 2020-08-23T15:47:57Z | |
dc.date.available | 2015-11-30 | |
dc.date.issued | 2015-11-30 | |
dc.identifier.uri | https://etd.library.vanderbilt.edu/etd-11192015-095602 | |
dc.identifier.uri | http://hdl.handle.net/1803/14630 | |
dc.description.abstract | 2-Amino-3-methylimidizo-[4,5-ʄ]-quinoline (IQ), a potent dietary mutagen, forms DNA adducts at the N² and C8-positions of 2ʹ-deoxyguanosine. IQ induces G to A transitions and G to T transversions in eukaryotic cells. The in vitro replication of the N²-dG-IQ and C8-dG-IQ adducts was examined by translesion DNA polymerases to determine their contributions to the observed mutagenic spectra. Our results implicate DNA polymerases kappa and eta in the error-free bypass of N2- and C8-dG-IQ adducts, respectively. In addition, DNA polymerase combinations eta/zeta and kappa/zeta are implicated in the mis-incorporation of A and T. | |
dc.format.mimetype | application/pdf | |
dc.subject | DNA Adducts | |
dc.subject | Translesion Synthesis | |
dc.subject | 2-Amino-3-methylimidazo-[4 | |
dc.subject | 5-f]-quinoline | |
dc.subject | Heterocyclic Amines | |
dc.subject | DNA Polymerases | |
dc.title | Translesion Synthesis of N2 and C8-Deoxyguanosine Adducts of the Dietary
Mutagen 2-Amino-3-methylimidazo-[4,5-f]-quinoline (IQ): Participation in Observed Mutagenic Spectra | |
dc.type | dissertation | |
dc.contributor.committeeMember | Martin Egli, Ph.D. | |
dc.contributor.committeeMember | Brandt F. Eichman | |
dc.contributor.committeeMember | Michael P. Stone | |
dc.type.material | text | |
thesis.degree.name | PHD | |
thesis.degree.level | dissertation | |
thesis.degree.discipline | Chemistry | |
thesis.degree.grantor | Vanderbilt University | |
local.embargo.terms | 2015-11-30 | |
local.embargo.lift | 2015-11-30 | |
dc.contributor.committeeChair | Carmelo J. Rizzo, Ph.D. | |