dc.creator | Xu, Wen | |
dc.date.accessioned | 2020-08-23T15:46:09Z | |
dc.date.available | 2010-12-01 | |
dc.date.issued | 2008-12-01 | |
dc.identifier.uri | https://etd.library.vanderbilt.edu/etd-11172008-114513 | |
dc.identifier.uri | http://hdl.handle.net/1803/14565 | |
dc.description.abstract | This dissertation involved the structural investigations of butadiene-derived N6,N6-deoxycytosine intrastrand cross-linked adduct and N3-deoxyuridine adducts in DNA by NMR or X-ray crystallography, respectively. Solution structure of the N6,N6-deoxycytosine intrastrand cross-link indicated that the butadiene lesion was oriented in the major groove and existed in two conformations with formation of different hydrogen bonds between the beta-OH and the 5' base pair T16 O4 or the 3' base pair T17 O4. Crystal structures of binary Dpo4-DNA complexes and ternary Dpo4-DNA-d(d)NTP complexes containing BD-N3-deoxyuridine adducts with the Sulfolobus solfataricus DNA Polymerase IV (Dpo4) were determined. This is the first time to study Dpo4 complexes containing non-planar adducts in the template. The resulted non-productive crystal structures suggested that steric effect plays a significant role in bypass of damaged DNA by trans-lesion polymerase Dpo4. | |
dc.format.mimetype | application/pdf | |
dc.subject | DNA adducts -- Structure | |
dc.subject | NMR | |
dc.subject | X-ray crystallography | |
dc.subject | structure | |
dc.subject | DNA -- Conformation | |
dc.subject | Butadiene | |
dc.title | Structural studies of butadiene-derived DNA adducts | |
dc.type | dissertation | |
dc.contributor.committeeMember | Carmelo J. Rizzo | |
dc.contributor.committeeMember | Jens Meiler | |
dc.contributor.committeeMember | Brandt F. Eichman | |
dc.contributor.committeeMember | Martin Egli | |
dc.type.material | text | |
thesis.degree.name | PHD | |
thesis.degree.level | dissertation | |
thesis.degree.discipline | Chemistry | |
thesis.degree.grantor | Vanderbilt University | |
local.embargo.terms | 2010-12-01 | |
local.embargo.lift | 2010-12-01 | |
dc.contributor.committeeChair | Michael.P.Stone | |