dc.creator | Gibbons, Hunter Ramsdell | |
dc.date.accessioned | 2020-08-22T21:09:10Z | |
dc.date.available | 2019-10-15 | |
dc.date.issued | 2019-10-15 | |
dc.identifier.uri | https://etd.library.vanderbilt.edu/etd-10032019-143733 | |
dc.identifier.uri | http://hdl.handle.net/1803/14264 | |
dc.description.abstract | Naïve CD4+ T cells polarize into many varied CD4+ T-helper (TH) cell subsets depending on the cytokine milieu present during T cell receptor stimulation. Each T cell subset produces a unique cytokine profile to defend against varied pathogens. The polarization of each T cell subset is regulated by unique transcription factors and noncoding elements to induce cytokine expression. The research detailed identifies a noncoding RNA, GATA3-AS1, which is necessary for the expression of GATA3 and TH2 cell polarization. Furthermore, we describe the repression of IFN-γ by super-enhancer disruption via bromodomain inhibitor JQ1. Our results lend novel insight into the regulation of hallmark genes by noncoding elements in T-helper cell polarization. | |
dc.format.mimetype | application/pdf | |
dc.subject | T-Helper | |
dc.subject | Noncoding RNA | |
dc.subject | Super-enhancer | |
dc.subject | cytokine | |
dc.subject | transcription | |
dc.subject | Immunoprecipitation | |
dc.title | T-Helper Polarization: Regulation by Noncoding RNA and Super-Enhancers | |
dc.type | dissertation | |
dc.contributor.committeeMember | Oliver McDonald | |
dc.contributor.committeeMember | Gregor Neuert | |
dc.contributor.committeeMember | James W Thomas | |
dc.contributor.committeeMember | Thomas Aune | |
dc.type.material | text | |
thesis.degree.name | PHD | |
thesis.degree.level | dissertation | |
thesis.degree.discipline | Microbiology and Immunology | |
thesis.degree.grantor | Vanderbilt University | |
local.embargo.terms | 2019-10-15 | |
local.embargo.lift | 2019-10-15 | |
dc.contributor.committeeChair | Amy Major | |