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Pro-apoptotic BID facilitates ATR function following replicative stress

dc.creatorLiu, Yang
dc.date.accessioned2020-08-22T20:51:53Z
dc.date.available2013-09-02
dc.date.issued2011-09-02
dc.identifier.urihttps://etd.library.vanderbilt.edu/etd-08242011-093413
dc.identifier.urihttp://hdl.handle.net/1803/13996
dc.description.abstractFirst identified as a pro-apoptotic member in the programmed cell death pathway, BH3 interacting domain death agonist (BID) has recently been demonstrated to play a role to maintain genomic integrity and execute DNA damage checkpoints following DNA damage treatments. To clarify the function of BID in the cellular response to DNA damage, especially the response to replicative stress induced DNA damage, I used genetic and biochemical tools to investigate the function and mechanism of BID in the Ataxia-telangiectasia mutated and RAD3-related (ATR)-mediated signaling pathways in this dissertation. BID is demonstrated to mediate the ATR-directed DNA damage response following replicative stress. BID interacts with ATR/ATRIP and RPA to facilitate ATR function in the DNA damage sensor complex. In a mouse model, Bid preserves the hematopoietic system following hydroxyurea-induced replicative stress. Overall, my studies demonstrate the function of BID in the ATR-mediated DNA damage pathway at multiple levels, including protein-protein interaction, signaling transduction, cell function, and animal model. The significance of the dual functions of BID in both DNA damage and cell death pathways will be discussed.
dc.format.mimetypeapplication/pdf
dc.subjectapoptosis
dc.subjectDNA damage
dc.subjectRPA
dc.subjectATR/ATRIP
dc.subjectBID
dc.subjectreplicative stress
dc.titlePro-apoptotic BID facilitates ATR function following replicative stress
dc.typedissertation
dc.contributor.committeeMemberLaura A. Lee
dc.contributor.committeeMemberElizabeth Yang
dc.contributor.committeeMemberDavid Cortez
dc.contributor.committeeMemberSandra S. Zinkel
dc.type.materialtext
thesis.degree.namePHD
thesis.degree.leveldissertation
thesis.degree.disciplineCell and Developmental Biology
thesis.degree.grantorVanderbilt University
local.embargo.terms2013-09-02
local.embargo.lift2013-09-02
dc.contributor.committeeChairKathleen L. Gould


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