dc.creator | Markham, Nicholas Owen | |
dc.date.accessioned | 2020-08-22T17:37:43Z | |
dc.date.available | 2014-07-26 | |
dc.date.issued | 2012-07-26 | |
dc.identifier.uri | https://etd.library.vanderbilt.edu/etd-07192012-173715 | |
dc.identifier.uri | http://hdl.handle.net/1803/13246 | |
dc.description.abstract | p120-catenin (p120) is a master regulator of cellular adherens junctions and is important for epithelial homeostasis, development, tumorigenesis, and metastasis. Relatively little is known about the different functions of p120 isoforms. During validation of a yeast two-hybrid screen using p120 as bait, I detected an isoform-specific interaction with Delta-Interacting Protein A (DIPA). In this study, I show that DIPA co-localizes and co-immunoprecipitates reciprocally with the long p120-1 isoform, but not the shorter p120-3 isoform, which lacks an N-terminal head domain. Also, p120-1 expression is required for the plasma membrane localization of DIPA. The p120 family members ARVCF, p0071, and δ-catenin have similar N-terminal regions to p120-1 and also bind to DIPA, suggesting that the interaction is highly conserved. These family members can recruit DIPA to the plasma membrane in the absence of p120-1. DIPA is the first protein discovered to selectively interact with isoform p120-1, and my data suggest that their relationship is potentially important for a novel, cell contact mediated function. | |
dc.format.mimetype | application/pdf | |
dc.subject | adherens junctions | |
dc.subject | DIPA | |
dc.subject | p120-catenin | |
dc.title | DIPA is a novel, isoform-specific binding partner of the tumor-associated p120 isoform 1 | |
dc.type | dissertation | |
dc.contributor.committeeMember | Albert B. Reynolds | |
dc.contributor.committeeMember | Scott W. Hiebert | |
dc.contributor.committeeMember | Christopher S. Williams | |
dc.contributor.committeeMember | David Cortez | |
dc.type.material | text | |
thesis.degree.name | PHD | |
thesis.degree.level | dissertation | |
thesis.degree.discipline | Cancer Biology | |
thesis.degree.grantor | Vanderbilt University | |
local.embargo.terms | 2014-07-26 | |
local.embargo.lift | 2014-07-26 | |
dc.contributor.committeeChair | Robert J. Coffey | |