• About
    • Login
    View Item 
    •   Institutional Repository Home
    • Electronic Theses and Dissertations
    • Electronic Theses and Dissertations
    • View Item
    •   Institutional Repository Home
    • Electronic Theses and Dissertations
    • Electronic Theses and Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of Institutional RepositoryCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsDepartmentThis CollectionBy Issue DateAuthorsTitlesSubjectsDepartment

    My Account

    LoginRegister

    Analyzing genome integrity during DNA replication using iPOND (isolation of Proteins On Nascent DNA)

    Sirbu, Bianca Maria
    : https://etd.library.vanderbilt.edu/etd-07122013-084538
    http://hdl.handle.net/1803/12944
    : 2013-07-12

    Abstract

    The in vivo analysis of dynamic events that protect genomic and epigenomic integrity during DNA replication has been a challenging task. The proteins that ensure the accurate inheritance of the genome act directly at replication forks. Unfortunately, tracking and purifying replication forks has been difficult in mammalian cells because the location of elongating forks is difficult to predict. I have developed the iPOND (isolation of Proteins On Nascent DNA) technique as a tool to isolate, analyze and discover proteins that localize to replication forks during active DNA replication and following conditions of DNA damage. The iPOND technique provides a useful tool to investigate the coordinated mechanisms that maintain the genome and epigenome through DNA replication. These pathways are perturbed in human diseases such as cancer.
    Show full item record

    Files in this item

    Icon
    Name:
    Sirbu.pdf
    Size:
    9.466Mb
    Format:
    PDF
    View/Open

    This item appears in the following collection(s):

    • Electronic Theses and Dissertations

    Connect with Vanderbilt Libraries

    Your Vanderbilt

    • Alumni
    • Current Students
    • Faculty & Staff
    • International Students
    • Media
    • Parents & Family
    • Prospective Students
    • Researchers
    • Sports Fans
    • Visitors & Neighbors

    Support the Jean and Alexander Heard Libraries

    Support the Library...Give Now

    Gifts to the Libraries support the learning and research needs of the entire Vanderbilt community. Learn more about giving to the Libraries.

    Become a Friend of the Libraries

    Quick Links

    • Hours
    • About
    • Employment
    • Staff Directory
    • Accessibility Services
    • Contact
    • Vanderbilt Home
    • Privacy Policy