dc.creator | Hu, Yunfeng | |
dc.date.accessioned | 2020-08-22T00:44:14Z | |
dc.date.available | 2009-05-20 | |
dc.date.issued | 2009-05-20 | |
dc.identifier.uri | https://etd.library.vanderbilt.edu/etd-05192009-102618 | |
dc.identifier.uri | http://hdl.handle.net/1803/12342 | |
dc.description.abstract | The biosyntheses of benzodiazepines and nitrosugar containing natural products in actinomycetes were investigated. The first biosynthetic gene cluster of a benzodiazepine natural product was identified in the genome of the anthramycin producer Streptomyces refuineus. The functional boundaries of this biosynthetic gene cluster were validated via a series of targeted gene disruptions and chemical complementation studies were utilized to elucidate aspects of the anthramycin biosynthetic pathway. In a separate study, heterologous expression of the anthramycin biosynthetic gene cluster demonstrated the completeness of the putative gene cluster and shed light on a potential temperature dependent regulation phenomenon. Lastly, the biosynthesis of nitrosugar natural products was investigated. A novel nitrososynthase was putatively identified in the previously sequenced biosynthetic gene cluster of everninomycin via comparative genomics and the biochemical function of this oxidase was demonstrated in the conversion a TDP-aminosugar to a TDP-nitrososugar via a hydroxylamine intermediate. | |
dc.format.mimetype | application/pdf | |
dc.subject | nitrosugar | |
dc.subject | benzodiazepines | |
dc.subject | biosynthesis | |
dc.subject | natural products | |
dc.title | Microbial biosynthesis of benzodiazepines and nitrosugar containing natural products | |
dc.type | dissertation | |
dc.contributor.committeeMember | David W. Wright | |
dc.contributor.committeeMember | Michael P. Stone | |
dc.contributor.committeeMember | Richard N. Armstrong | |
dc.type.material | text | |
thesis.degree.name | PHD | |
thesis.degree.level | dissertation | |
thesis.degree.discipline | Chemistry | |
thesis.degree.grantor | Vanderbilt University | |
local.embargo.terms | 2009-05-20 | |
local.embargo.lift | 2009-05-20 | |
dc.contributor.committeeChair | Brian O. Bachmann | |