• About
    • Login
    View Item 
    •   Institutional Repository Home
    • Electronic Theses and Dissertations
    • Electronic Theses and Dissertations
    • View Item
    •   Institutional Repository Home
    • Electronic Theses and Dissertations
    • Electronic Theses and Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of Institutional RepositoryCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsDepartmentThis CollectionBy Issue DateAuthorsTitlesSubjectsDepartment

    My Account

    LoginRegister

    The contribution of RAS function to transformation of the colonic epithelium: functional differences, similarities, and cooperation between RAS family members

    Keller, Jeffrey Wayne
    : https://etd.library.vanderbilt.edu/etd-05162006-153153
    http://hdl.handle.net/1803/12321
    : 2006-06-01

    Abstract

    Constitutively activating mutations of members of the RAS family of small G proteins provide an important oncogenic contribution to a significant percentage of human cancers. Despite nearly 25 years of investigation, important questions about the function of these proteins and their relationships to one another remain unresolved. We have undertaken a series of studies to address some of these questions and herein report novel findings regarding the relationship of these family members to one another and their respective contributions to the progression of human disease. Briefly, we examine the uniquely potent oncogenic contribution of K-RAS to cell lines derived from human tumors and identify some previously unappreciated findings regarding the mechanisms by which this protein promotes tumorigenesis. Interestingly, we present data to support that K-RAS requires the activity of another family member, NRAS to realize its full oncogenic potential in certain contexts, and that represents the first biochemical evidence of a relationship between RAS family members. We additionally challenge earlier notions about effector pathways long thought to be essential for RAS-driven transformation, as well as disprove an accepted and widespread assay for measuring the activity of RAS proteins.
    Show full item record

    Files in this item

    Icon
    Name:
    KELLER_DISSERTATION_FINAL.pdf
    Size:
    5.643Mb
    Format:
    PDF
    View/Open

    This item appears in the following collection(s):

    • Electronic Theses and Dissertations

    Connect with Vanderbilt Libraries

    Your Vanderbilt

    • Alumni
    • Current Students
    • Faculty & Staff
    • International Students
    • Media
    • Parents & Family
    • Prospective Students
    • Researchers
    • Sports Fans
    • Visitors & Neighbors

    Support the Jean and Alexander Heard Libraries

    Support the Library...Give Now

    Gifts to the Libraries support the learning and research needs of the entire Vanderbilt community. Learn more about giving to the Libraries.

    Become a Friend of the Libraries

    Quick Links

    • Hours
    • About
    • Employment
    • Staff Directory
    • Accessibility Services
    • Contact
    • Vanderbilt Home
    • Privacy Policy