• About
    • Login
    View Item 
    •   Institutional Repository Home
    • Electronic Theses and Dissertations
    • Electronic Theses and Dissertations
    • View Item
    •   Institutional Repository Home
    • Electronic Theses and Dissertations
    • Electronic Theses and Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of Institutional RepositoryCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsDepartmentThis CollectionBy Issue DateAuthorsTitlesSubjectsDepartment

    My Account

    LoginRegister

    Combining laser capture microdissection and MALDI mass spectrometry for tissue protein profiling: methodology development and clinical applications

    Xu, Baogang Jonathan
    : https://etd.library.vanderbilt.edu/etd-03092005-132210
    http://hdl.handle.net/1803/10706
    : 2005-03-09

    Abstract

    The combination of laser capture microdissection (LCM) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) for protein profiling from specific cells of interest within heterogeneous tissue is presented in this thesis. The methodology of direct analysis of laser capture microdissected cells using MALDI MS is described. The sensitivity and reproducibility of this approach were reported. High quality protein expression profiles at the mass range of 2,000 to 50,000 Da are obtained. As the protocols were optimized, three clinical applications were studied: 1) protein biomarkers of normal and neoplastic mouse colon, 2) proteomic patterns of glomerulosclerosis and their use for the prediction of sclerosis progression, and 3) proteomic patterns of human breast tissue aimed at the molecular distinction of breast cancer and normal mammary tissue with classification of in situ tumor and invasive tumor. Statistically significant protein markers were identified for each of the tissue groups by amino acid sequence analysis. Immunostaining studies further confirmed the differential expression of some of these protein markers. In summary, LCM combined with MALDI MS is a sensitive, accurate, fast and reliable method to discover protein biomarkers which can aid in disease diagnosis, discovery of drug targets and advance the understanding of underlying mechanisms of diseases.
    Show full item record

    Files in this item

    Icon
    Name:
    Xu_Thesis.pdf
    Size:
    5.268Mb
    Format:
    PDF
    View/Open

    This item appears in the following collection(s):

    • Electronic Theses and Dissertations

    Connect with Vanderbilt Libraries

    Your Vanderbilt

    • Alumni
    • Current Students
    • Faculty & Staff
    • International Students
    • Media
    • Parents & Family
    • Prospective Students
    • Researchers
    • Sports Fans
    • Visitors & Neighbors

    Support the Jean and Alexander Heard Libraries

    Support the Library...Give Now

    Gifts to the Libraries support the learning and research needs of the entire Vanderbilt community. Learn more about giving to the Libraries.

    Become a Friend of the Libraries

    Quick Links

    • Hours
    • About
    • Employment
    • Staff Directory
    • Accessibility Services
    • Contact
    • Vanderbilt Home
    • Privacy Policy