dc.creator | Huang, Chen | |
dc.date.accessioned | 2020-08-21T21:03:13Z | |
dc.date.available | 2018-03-31 | |
dc.date.issued | 2017-03-31 | |
dc.identifier.uri | https://etd.library.vanderbilt.edu/etd-02222017-163339 | |
dc.identifier.uri | http://hdl.handle.net/1803/10611 | |
dc.description.abstract | Both type I and type II diabetes are related to β-cell defects in the pancreatic islet of Langerhans. Deriving β-cells from stem cells and other mature cell types provides an important cell source for transplantation-based therapy to treat diabetes. Mechanistic studies of β-cell maturation and functional maintenance are crucial in providing novel insights for the generation of glucose-responsive and long-term sustainable β-cells. In this study, I found that two gene/gene families, synaptotagmin IV (Syt4) and Myt factors are essential to promote β-cell maturation and functional maintenance. Mouse studies provided evidence that Syt4 modulates insulin Ca2+ vesicle sensitivity to facilitate β-cell maturation the neonatal stage. Loss of Syt4 in mice resulted in Ca2+ hypersensitivity of insulin vesicles in β-cells and compromised glucose-stimulated insulin secretion. Conversely, Syt4 overexpression reduced insulin Ca2+ vesicle sensitivity and established the mature insulin secretion profile in the newborn β-cells. Moreover, Myt factors are essential to generate functional β-cells. Postnatal β-cells in a Myt knockout mouse model were characterized by functional failure, cell apoptosis and loss of mature β-cell identity. Loss of Myt factors in β-cells disrupted the expression of genes involved in insulin secretion, β-cell survival and identity maintenance. These combined results suggest that Syt4 and Myt factors can be exploited as molecular targets to promote β-cell maturity and long-term functional maintenance for better clinical β-cell regeneration. | |
dc.format.mimetype | application/pdf | |
dc.subject | Synaptotagmin IV | |
dc.subject | Myt factors | |
dc.subject | β-cell | |
dc.subject | diabetes | |
dc.subject | mouse | |
dc.subject | development | |
dc.title | Synaptotagmin IV and Myt factors promote β-cell functional
maturation and maintenance | |
dc.type | dissertation | |
dc.contributor.committeeMember | Guoqiang Gu | |
dc.contributor.committeeMember | Tony Weil | |
dc.contributor.committeeMember | David Bader | |
dc.contributor.committeeMember | Maureen Gannon | |
dc.type.material | text | |
thesis.degree.name | PHD | |
thesis.degree.level | dissertation | |
thesis.degree.discipline | Cell and Developmental Biology | |
thesis.degree.grantor | Vanderbilt University | |
local.embargo.terms | 2018-03-31 | |
local.embargo.lift | 2018-03-31 | |
dc.contributor.committeeChair | Chin Chiang | |