Show simple item record

Identification of Novel Determinants of HIV-1 Uncoating in the Capsid Protein

dc.creatorYang, Ruifeng
dc.description.abstractUncoating of the viral core following penetration into the target cell represents a fundamentally obscure step in the HIV-1 life cycle. Our laboratory has previously reported that mutations in the CA protein that positively and negatively alter the stability of the viral capsid shell result in impaired HIV-1 infectivity, suggesting that the HIV-1 core is optimally balanced for proper uncoating in target cells. To identify additional determinants contributing to optimal HIV-1 capsid stability in CA, I identified second-site suppressors of HIV-1 uncoating mutants by serial passage. My results indicate that CA determinants outside the CypA-binding loop can modulate the dependence of HIV-1 infection on CypA. Further, based on the recently reported full-length HIV-1 hexamer structure, my data provide novel genetic evidence of HIV-1 CA direct intersubunit interactions. My results also suggest that cellular factors are involved in HIV-1 uncoating. A detailed understanding of the mechanism and determinants of HIV-1 uncoating should facilitate the development of novel antiviral therapies targeting this key step in HIV-1 infection.
dc.titleIdentification of Novel Determinants of HIV-1 Uncoating in the Capsid Protein
dc.contributor.committeeMemberW. Gray Jerome
dc.contributor.committeeMemberGerald Stubbs
dc.contributor.committeeMemberMark Denison
dc.contributor.committeeMemberChristopher Aiken
dc.type.materialtext and Immunology University
dc.contributor.committeeChairRich D'Aquila

Files in this item


This item appears in the following Collection(s)

Show simple item record