• About
    • Login
    View Item 
    •   Institutional Repository Home
    • Electronic Theses and Dissertations
    • Electronic Theses and Dissertations
    • View Item
    •   Institutional Repository Home
    • Electronic Theses and Dissertations
    • Electronic Theses and Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of Institutional RepositoryCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsDepartmentThis CollectionBy Issue DateAuthorsTitlesSubjectsDepartment

    My Account

    LoginRegister

    RNA Recognition by the Histone Demethylase LSD1 and Proteinaceous RNase P: Characterization of the Binding of Highly Structured RNAs by Enzyme Complexes

    Martin, William Jay
    : https://etd.library.vanderbilt.edu/etd-02082018-150116
    http://hdl.handle.net/1803/10546
    : 2018-02-13

    Abstract

    This study investigates the recognition of structured RNAs by two essential enzymes, proteinaceous RNase P (PRORP) and lysine-specific demethylase-1 (LSD1). PRORP binds and cleaves human mitochondrial precursor tRNAs in a fundamental step for the generation of mature mitochondrial transcripts. The enzyme recognizes specific structured domains of the pre-tRNAs in a manner similar to but distinct from the unrelated nuclear RNase P complex. In order to move towards a crystal structure of the PRORP-RNA complex, minimal constructs were generated and biochemically validated with binding studies and in vitro activity assays and used in crystallization studies. LSD1 modulates gene expression through enzymatic histone demethylation and also serves as a protein scaffold in various large protein complexes. The long noncoding RNA (lncRNA) telomeric repeat-containing RNA (TERRA) has previously been demonstrated to recruit LSD1 to deprotected telomeres where it promotes the recruitment of the nuclease MRE11. Here, it is shown that LSD1 specifically recognizes the G-quadruplex structure formed by TERRA and other RNAs and a G-quadruplex RNA binding region is identified in the regulatory SWIRM domain of LSD1. Together, these studies advance our understanding of the role of structured RNAs in RNA/protein interactions.
    Show full item record

    Files in this item

    Icon
    Name:
    Martin.pdf
    Size:
    3.339Mb
    Format:
    PDF
    View/Open

    This item appears in the following collection(s):

    • Electronic Theses and Dissertations

    Connect with Vanderbilt Libraries

    Your Vanderbilt

    • Alumni
    • Current Students
    • Faculty & Staff
    • International Students
    • Media
    • Parents & Family
    • Prospective Students
    • Researchers
    • Sports Fans
    • Visitors & Neighbors

    Support the Jean and Alexander Heard Libraries

    Support the Library...Give Now

    Gifts to the Libraries support the learning and research needs of the entire Vanderbilt community. Learn more about giving to the Libraries.

    Become a Friend of the Libraries

    Quick Links

    • Hours
    • About
    • Employment
    • Staff Directory
    • Accessibility Services
    • Contact
    • Vanderbilt Home
    • Privacy Policy