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Now showing items 11-20 of 24
Mechanisms by which calcium regulates the human cardiac voltage-gated sodium channel hH1
(2005-08-12)
The function of the human cardiac voltage-gated sodium channel hH1 is regulated in part by an EF-hand in its C-terminal cytoplasmic domain. The binding of calcium to the EF-hand results in calmodulin-independent, ...
Biochemical and structural analysis of the p58C and p68N domains of DNA polymerase alpha/primase
(2008-07-28)
The replication of DNA occurs through a complex series of steps involving the coordinated action of many proteins. DNA polymerase alpha/primase (pol-prim) is a critical DNA replication factor that synthesizes short RNA-DNA ...
DNA Lesions as Cellular Poisons of Topoisomerase II-alpha
(2005-11-02)
Topoisomerase II is an essential enzyme that controls DNA topology. This enzyme generates a protein-linked DNA break as a catalytic intermediate. This intermediate, known as the cleavage complex, is present in very low ...
Quinone Metabolites of Environmental Toxins Poison Topoisomerase IIalpha
(2007-03-31)
Topoisomerases play critical roles in maintaining DNA topology during cellular processes such as DNA replication in eukaryotes. Movement of the replication machinery through the double helix induces positive supercoiling ...
Regulation of ATR Signaling by CINP and RPA
(2009-05-28)
Genetic instability is a hallmark of cancer. The ATR-ATRIP complex functions at the apex of a cell cycle checkpoint signaling pathway that is critical during every cell division cycle to maintain genome integrity. ATR ...
Regulation of Fibronectin Assembly by PLC-gamma1
(2009-04-21)
Phospholipase C-γ1 (PLC-γ1) mediates cell adhesion and migration through an undefined mechanism. Here, we examine the role of PLC-γ1 in cell-matrix adhesion in a hanging drop assay of cell aggregation. Plcg1 Null (-/-) ...
Dynamic, Structural, and Mechanistic Study of Glutathione Transferases
(2006-06-30)
This project involved the investigation of both the dynamic features of the dimer interface of the Mu class GSH transferase rGSTM1-1, as well as, the structural and mechanistic characteristics of HCCA Isomerase, an enzyme ...
In vivo characterization of the role of histone deacetylase 3 in metabolic and transcriptional regulation
(2008-05-30)
Histone deacetylase 3 (HDAC3) is the enzymatic component of transcriptional repression complexes recruited by the nuclear hormone receptors. Inactivation of HDAC3 in cancer cell lines triggered apoptosis, and removal of ...
Discovery and characterization of the mTOR-p73 signaling axis in human cancer
(2009-06-08)
p73, a transcription factor that plays essential roles during development and tumor suppression, shares significant sequence identity with p53. However, p53 and p73 are regulated by different pathways, and p73 is not ...
Human Topoisomerases and DNA Geometry: Putting a Positive Twist on Enzyme Action
(2006-04-12)
HUMAN TOPOISOMERASES AND DNA GEOMETRY: PUTTING A POSITIVE TWIST ON ENZYME ACTION
AMY KATHLEEN MCCLENDON
Topoisomerases play critical roles in maintaining DNA topology during cellular processes such as DNA replication ...