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Identification of Potential Virulence Factors in the Model Strain Acinetobacter baumannii A118

dc.contributor.authorRamirez, Maria S.
dc.contributor.authorPenwell, William F.
dc.contributor.authorTraglia, German M.
dc.contributor.authorZimbler, Daniel L.
dc.contributor.authorGaddy, Jennifer A.
dc.contributor.authorNikolaidis, Nikolas
dc.contributor.authorArivett, Brock A.
dc.contributor.authorAdams, Mark D.
dc.contributor.authorBonomo, Robert A.
dc.contributor.authorActis, Luis A.
dc.contributor.authorTolmasky, Marcelo E.
dc.identifier.citationRamirez MS, Penwell WF, Traglia GM, Zimbler DL, Gaddy JA, Nikolaidis N, Arivett BA, Adams MD, Bonomo RA, Actis LA and Tolmasky ME (2019) Identification of Potential Virulence Factors in the Model Strain Acinetobacter baumannii A118. Front. Microbiol. 10:1599. doi: 10.3389/fmicb.2019.01599en_US
dc.description.abstractAcinetobacter baumannii A118, a strain isolated from the blood of an infected patient, is naturally competent and unlike most clinical strains, is susceptible to a variety of different antibiotics including those usually used for selection in genetic manipulations. These characteristics make strain A118 a convenient model for genetic studies of A. baumannii. To identify potential virulence factors, its complete genome was analyzed and compared to other A. baumannii genomes. A. baumannii A118 includes gene clusters coding for the acinetobactin and baumannoferrin iron acquisition systems. Iron-regulated expression of the BauA outer membrane receptor for ferric-acinetobactin complexes was confirmed as well as the utilization of acinetobactin. A. baumannii A118 also possesses the feoABC genes, which code for the main bacterial ferrous uptake system. The functionality of baumannoferrin was suggested by the ability of A. baumannii A118 culture supernatants to cross feed an indicator BauA-deficient strain plated on iron-limiting media. A. baumannii A118 behaved as non-motile but included the csuA/BABCDE chaperone-usher pilus assembly operon and produced biofilms on polystyrene and glass surfaces. While a known capsular polysaccharide (K) locus was identified, the outer core polysaccharide (OC) locus, which belongs to group B, showed differences with available sequences. Our results show that despite being susceptible to most antibiotics, strain A118 conserves known virulence-related traits enhancing its value as model to study A. baumannii pathogenicity.en_US
dc.description.sponsorshipThis study was supported by Public Health Service grants 2R15AI047115 (to MT), AI070174 (to LA), R01AI00560, R01AI0AI063517, R21AI11458, and R01AI072219 (to RB), and SC3G-M125556 (to MR) from the National Institutes of Health, award 1I01BX001974 (to RB) from the BLRDS (Veterans Administration Office of Research and Development), and award VISN10 (to RB) from the Geriatric Research and Educational and Clinical Center. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.en_US
dc.rightsCopyright © 2019 Ramirez, Penwell, Traglia, Zimbler, Gaddy, Nikolaidis, Arivett, Adams, Bonomo, Actis and Tolmasky. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
dc.subjectvirulence factorsen_US
dc.subjecthospital infectionen_US
dc.subjectcommunity infectionen_US
dc.titleIdentification of Potential Virulence Factors in the Model Strain Acinetobacter baumannii A118en_US

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