Aminoclay Nanoparticles Induce Anti-Inflammatory Dendritic Cells to Attenuate LPS-Elicited Pro-Inflammatory Immune Responses
dc.contributor.author | Park, Hyun Jung | |
dc.contributor.author | Lee, Sung Won | |
dc.contributor.author | Song, Jae Geun | |
dc.contributor.author | Van Kaer, Luc | |
dc.contributor.author | Cheon, Jae Hee | |
dc.contributor.author | Lim, Soo-Jeong | |
dc.contributor.author | Han, Hyo-Kyung | |
dc.contributor.author | Hong, Seokmann | |
dc.date.accessioned | 2023-01-31T21:07:17Z | |
dc.date.available | 2023-01-31T21:07:17Z | |
dc.date.issued | 2022-12-09 | |
dc.identifier.citation | Park, H.J.; Lee, S.W.; Song, J.G.; Van Kaer, L.; Cheon, J.H.; Lim, S.-J.; Han, H.-K.; Hong, S. Aminoclay Nanoparticles Induce Anti-Inflammatory Dendritic Cells to Attenuate LPS-Elicited Pro-Inflammatory Immune Responses. Molecules 2022, 27, 8743. https://doi.org/10.3390/ molecules27248743 | en_US |
dc.identifier.other | eISSN : 1420-3049 | |
dc.identifier.uri | http://hdl.handle.net/1803/17968 | |
dc.description.abstract | Although 3-aminopropyl functionalized magnesium phyllosilicate nanoparticles (hereafter aminoclay nanoparticles, ACNs) are well-known nanomaterials employed as drug carriers, their effects on immune cells remain unclear. To address this issue, we explored murine dendritic cells (DCs) as these cells belong to the innate arm of the immune system and function as antigen-presenting cells to elicit adaptive immune responses. We examined the in vitro effects of ACNs on DCs isolated from B6 mice. ACN treatment significantly down-regulated the expression of inflammasome-related markers, including NLRP3, caspase-1, and IL1 beta. The ACNs-induced anti-inflammatory DC phenotype was further confirmed by down-regulation of the AKT/mTOR/HIF1 alpha signaling pathway. Such anti-inflammatory effects of ACNs on DCs occurred independently of DC subtypes. To document the effects of ACNs on DCs more clearly, we examined their anti-inflammatory effects on lipopolysaccharide (LPS)-activated DCs. As expected, excessive inflammatory responses (increased mitochondrial ROS and Th1-type cytokines such as IL12 and IL1 beta) of LPS-activated DCs were dramatically attenuated by ACN treatment. Furthermore, ACNs down-regulated IFN gamma production by antigen-specific CD4(+) T cells, which is consistent with a reduced inflammatory phenotype of DCs. Overall, our results provide support for employing ACNs as drug delivery materials with therapeutic potential to control inflammatory disorders | en_US |
dc.description.sponsorship | This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2021R1I1A1A01054418 to S.W.L.; NRF-2021R1I1A1A01051465 to H.J.P.; NRF-2019R1A2C1009926 and NRF-2022R1A2C1009590 to S.H.). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Molecules | en_US |
dc.rights.uri | © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). | |
dc.rights.uri | https://www.mdpi.com/1420-3049/27/24/8743 | |
dc.subject | aminoclay | en_US |
dc.subject | dendritic cells | en_US |
dc.subject | lipopolysaccharide (LPS) | en_US |
dc.subject | IL1β | en_US |
dc.title | Aminoclay Nanoparticles Induce Anti-Inflammatory Dendritic Cells to Attenuate LPS-Elicited Pro-Inflammatory Immune Responses | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.3390/molecules27248743 |
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