dc.creator | Dale, Bethany Lyn | |
dc.date.accessioned | 2020-08-24T11:49:50Z | |
dc.date.available | 2020-04-30 | |
dc.date.issued | 2019-04-30 | |
dc.identifier.uri | https://etd.library.vanderbilt.edu/etd-04252019-201237 | |
dc.identifier.uri | http://hdl.handle.net/1803/15413 | |
dc.description.abstract | The role of T and B cells of the adaptive immune have been implicated in hypertension, but the mechanisms by which they produce a coordinated response is unknown. T follicular helper (Tfh) cells that produce interleukin 21 (IL- 21), can act upstream of IL-17A and interferon gamma (IFNg) which are known to promote hypertension, as well as promote germinal center (GC) B cell responses leading to immunoglobulin (Ig) production. The role of IL-21 and Tfh cells were investigated using two mouse models, Il21-/- mice, and Bcl6flox/floxTgCD4cre mice, as well as pharmacological intervention after the onset of hypertension using therapeutic grade neutralizing antibodies to murine IL-21. The impact genetic mouse lines or therapeutic intervention had on hypertension and end-organ damage were assessed by blood pressure measurements, vascular reactivity studies, vascular inflammation, and aortic remodeling. The results of these studies were associated with hypertension in humans through measurement of T cell production of IL-21 and IL-17A from peripheral blood. Additionally, through the use of mass cytometry and cytometry by time of flight (CyTOF), the ability to identify and detect previously unknown producing cells of IL-17A and INFg was accomplished. | |
dc.format.mimetype | application/pdf | |
dc.subject | hypertension | |
dc.subject | vascular dysfunction | |
dc.subject | interleukin 21 | |
dc.subject | T follicular helper | |
dc.subject | blood pressure | |
dc.title | The Influence of Interleukin 21 and T Follicular Helper Cells in Hypertension and Vascular Dysfunction | |
dc.type | dissertation | |
dc.contributor.committeeMember | Jeffrey Rathmell | |
dc.contributor.committeeMember | Raymond Harris | |
dc.contributor.committeeMember | Ambra Pozzi | |
dc.contributor.committeeMember | Jonathan Irish | |
dc.type.material | text | |
thesis.degree.name | PHD | |
thesis.degree.level | dissertation | |
thesis.degree.discipline | Molecular Physiology and Biophysics | |
thesis.degree.grantor | Vanderbilt University | |
local.embargo.terms | 2020-04-30 | |
local.embargo.lift | 2020-04-30 | |
dc.contributor.committeeChair | Alyssa Hasty | |