dc.creator | Trantum, Joshua Robert | |
dc.date.accessioned | 2020-08-23T16:18:41Z | |
dc.date.available | 2012-12-16 | |
dc.date.issued | 2010-12-16 | |
dc.identifier.uri | https://etd.library.vanderbilt.edu/etd-12102010-133635 | |
dc.identifier.uri | http://hdl.handle.net/1803/15243 | |
dc.description.abstract | More than 700,000 deaths are attributed to malarial infection annually in the rural areas of Sub-Saharan Africa. Effective and reliable early detection is inadequate with currently available technology. Development of a low-cost, sensitive and simple-to-use diagnostic assay appropriate for the point-of-care setting is a critical component of malaria containment efforts. This work has demonstrated a diagnostic assay based on the same mechanism that causes a ring to form in an evaporating coffee drop. This novel method for noninstrumented signal visualization is potentially well-suited for low-resource applications that require a simple-to-use, low cost method for pathogen detection. After exploring and optimizing basic design parameters, we demonstrated proof-of-principle for malaria testing by detecting poly-l-histidine, a biomolecular mimic of pfHRP2. The proposed assay was found to have a limit of detection in the mid-nanomolar range, approximately one order of magnitude higher than the clinically relevant range. | |
dc.format.mimetype | application/pdf | |
dc.subject | microparticle | |
dc.subject | coffee stain phenomenon | |
dc.subject | malaria | |
dc.subject | malaria diagnostic | |
dc.subject | low resource | |
dc.subject | diagnostic | |
dc.subject | diagnostic assay | |
dc.subject | low resource diagnostic assay | |
dc.subject | coffee ring phenomenon | |
dc.subject | nanoparticle | |
dc.subject | radial flow | |
dc.title | Low-resource diagnostic assay based on disruption of radial flow in an evaporating drop | |
dc.type | thesis | |
dc.contributor.committeeMember | David W. Wright | |
dc.type.material | text | |
thesis.degree.name | MS | |
thesis.degree.level | thesis | |
thesis.degree.discipline | Biomedical Engineering | |
thesis.degree.grantor | Vanderbilt University | |
local.embargo.terms | 2012-12-16 | |
local.embargo.lift | 2012-12-16 | |
dc.contributor.committeeChair | Frederick R. Haselton | |