dc.creator | Carboneau, Bethany Ann | |
dc.date.accessioned | 2020-08-22T20:47:01Z | |
dc.date.available | 2017-08-16 | |
dc.date.issued | 2017-08-16 | |
dc.identifier.uri | https://etd.library.vanderbilt.edu/etd-08152017-102047 | |
dc.identifier.uri | http://hdl.handle.net/1803/13906 | |
dc.description.abstract | Type 2 diabetes is a major healthcare concern and is characterized by chronic hyperglycemia and low-grade inflammation. Hyperglycemia and systemic inflammation can induce the production of Prostaglandin E2 (PGE2) in islets. PGE2 signals through its four receptors, termed E-Prostanoid (EP) 1-4, to modulate many physiological functions, including insulin secretion and systemic inflammation. EP3 and EP4 often play opposing roles due to signaling through different G proteins, resulting in Gi inhibition (EP3) or Gs stimulation (EP4) of adenylyl cyclase. Previous work from our group revealed that Ptger4 (EP4) is increased while Ptger3 (EP3) is decreased in a mouse model of enhanced beta-cell proliferation and survival. Additional evidence from our lab suggests that EP3 inhibits mouse beta-cell proliferation in the setting of insulin resistance. Using ex vivo assays, we have determined that EP3 and EP4 play opposing roles in regulating beta-cell proliferation and survival in mouse and human islets: EP3 inhibits beta-cell proliferation via inhibition of PLC-gamma1 and enhances beta-cell death whereas EP4 activates beta-cell proliferation and promotes beta-cell survival in a PKA-dependent mechanism. | |
dc.format.mimetype | application/pdf | |
dc.subject | pancreatic beta cell | |
dc.subject | prostaglandins | |
dc.subject | proliferation | |
dc.subject | cell death | |
dc.subject | beta-cell mass | |
dc.title | Regulation of Beta-Cell Mass Expansion by Prostaglandin E2 Signaling | |
dc.type | dissertation | |
dc.contributor.committeeMember | Dr. Wenbiao Chen | |
dc.contributor.committeeMember | Dr. Roger Colbran | |
dc.contributor.committeeMember | Dr. Richard Breyer | |
dc.contributor.committeeMember | Dr. Laura Dugan | |
dc.type.material | text | |
thesis.degree.name | PHD | |
thesis.degree.level | dissertation | |
thesis.degree.discipline | Molecular Physiology and Biophysics | |
thesis.degree.grantor | Vanderbilt University | |
local.embargo.terms | 2017-08-16 | |
local.embargo.lift | 2017-08-16 | |
dc.contributor.committeeChair | Dr. David Jacobson | |