dc.creator | Grimaldi, Ashley Dyan | |
dc.date.accessioned | 2020-08-22T17:08:22Z | |
dc.date.available | 2016-06-20 | |
dc.date.issued | 2014-06-20 | |
dc.identifier.uri | https://etd.library.vanderbilt.edu/etd-06182014-172653 | |
dc.identifier.uri | http://hdl.handle.net/1803/12620 | |
dc.description.abstract | Microtubule plus-end tracking proteins (+TIPs) are a specialized group of microtubule-associated proteins (MAPs) that preferentially localize to microtubule (MT) plus-ends. This unique localization is thought to be due to +TIP recognition of a unique structural or chemical feature found only at the plus-ends of MTs, such as the GTP- tubulin cap. End-binding proteins (EBs) are considered the master regulators of the +TIP network; however, whether EBs are regulated by other +TIP proteins is not well understood. Therefore, a study of the regulation of EB proteins by CLASPs (CLIP-associated proteins), another family of +TIPs, was undertaken.
In cells lacking CLASPs, EB binding at MTs is significantly altered: both EB1 and EB3 relocalized to the MT lattice in addition to their characteristic plus-end localization. This study reveals that CLASPs likely function during MT polymerization to support efficient GTP-hydrolysis and thus promote specific EB plus-end localization. This work establishes CLASPs as crucial determinants of proper EB localization at MTs in cells. | |
dc.format.mimetype | application/pdf | |
dc.subject | MTs | |
dc.subject | CLASPs | |
dc.subject | Microtubule | |
dc.subject | EBs | |
dc.subject | +TIP Complex | |
dc.subject | End-Binding | |
dc.title | A Role for CLASPs in the Regulation of the Major Microtubule +TIP Complex Organizers, End-Binding Proteins | |
dc.type | dissertation | |
dc.contributor.committeeMember | Matthew Tyska | |
dc.contributor.committeeMember | Ryoma Ohi | |
dc.contributor.committeeMember | Matthew Lang | |
dc.type.material | text | |
thesis.degree.name | PHD | |
thesis.degree.level | dissertation | |
thesis.degree.discipline | Cell and Developmental Biology | |
thesis.degree.grantor | Vanderbilt University | |
local.embargo.terms | 2016-06-20 | |
local.embargo.lift | 2016-06-20 | |
dc.contributor.committeeChair | Laura Lee | |