dc.creator | Guckes, Kirsten Raquel | |
dc.date.accessioned | 2020-08-21T21:03:18Z | |
dc.date.available | 2019-02-23 | |
dc.date.issued | 2017-02-23 | |
dc.identifier.uri | https://etd.library.vanderbilt.edu/etd-02232017-114830 | |
dc.identifier.uri | http://hdl.handle.net/1803/10614 | |
dc.description.abstract | Bacteria use two-component system (TCS) signaling to sense and respond to their ever-changing surroundings. While most TCSs have been reported to interact solely as a cognate partner pair, one sensor kinase phosphorylating and dephosphorylating one response regulator, interactions between two-component systems grant uropathogenic E. coli the ability to finely calibrate responses to fluctuating environmental cues. Specifically, the QseBC and PmrAB two-component systems interact at both the post-translational, as well as the transcriptional level, to mediate a response to the signal ferric iron. The PmrB sensor kinase is able to phosphorylate its cognate regulator, PmrA, and non-cognate partner, QseB, in response to a ferric iron signal. Subsequent to ferric iron-mediated phosphorylation, both PmrA and QseB coordinate to regulate genes that allow the bacteria to become more tolerant to the antibiotic polymyxin B. Not only do QseBC-PmrAB interactions drive important antibiotic tolerance mechanisms, but they also provide a platform to investigate a phenomenon largely unobserved in TCS biology. | |
dc.format.mimetype | application/pdf | |
dc.subject | Bacteria | |
dc.subject | Two-component System | |
dc.subject | Uropathogenic E. coli | |
dc.subject | Gene regulation | |
dc.title | Investigating QseBC and PmrAB two-component system cross-interactions | |
dc.type | dissertation | |
dc.contributor.committeeMember | Oscar Gomez | |
dc.contributor.committeeMember | Thomas Stricker | |
dc.contributor.committeeMember | Maria Hadjifrangiskou | |
dc.contributor.committeeMember | Jonathan Irish | |
dc.type.material | text | |
thesis.degree.name | PHD | |
thesis.degree.level | dissertation | |
thesis.degree.discipline | Microbiology and Immunology | |
thesis.degree.grantor | Vanderbilt University | |
local.embargo.terms | 2019-02-23 | |
local.embargo.lift | 2019-02-23 | |
dc.contributor.committeeChair | Eric Skaar | |