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Loss of Function of Colgant1 Disrupts Collagen Post-translationa Modification and Causes Musculoskeletal Defects

dc.contributor.authorGeister, Krista A.
dc.contributor.authorLopez-Jimenez, Alberto Jose
dc.contributor.authorHoughtaling, Scott
dc.contributor.authorHo, Tzu-Hua
dc.contributor.authorVanacore, Roberto
dc.contributor.authorBeier, David R.
dc.identifier.citationKrista A. Geister, Alberto Jose Lopez-Jimenez, Scott Houghtaling, Tzu-Hua Ho, Roberto Vanacore, David R. Beier, Disease Models & Mechanisms 2019 12: dmm037176 doi: 10.1242/dmm.037176 Published 17 June 2019en_US
dc.description.abstractIn a screen for organogenesis defects in N-ethyl-N-nitrosourea (ENU)-induced mutant mice, we discovered a line carrying a mutation in Colgaft1 [collagen beta(1-O)galactosyltransferase type 1], which is required for proper galactosylation of hydroxylysine residues in a number of collagens. Colgaft1 mutant embryos have not been previously characterized; here, we show that they exhibit skeletal and muscular defects. Analysis of mutant-derived embryonic fibroblasts reveals that COLGALT1 acts on collagen IV and VI, and, while collagen VI appears stable and its secretion is not affected, collagen IV accumulates inside of cells and within the extracellular matrix, possibly due to instability and increased degradation. We also generated mutant zebrafish that do not express the duplicated orthologs of mammalian Colgaft1. The double-homozygote mutants have muscle defects; they are viable through the larvae stage but do not survive to 10 days post-fertilization. We hypothesize that the Colgaft1 mutant could serve as a model of a human connective tissue disorder and/or congenital muscular dystrophy or myopathy.en_US
dc.description.sponsorshipThis work was supported in part by the National Institutes of Health (HD036404 to D.R.B. and DK099467 to R.V.)en_US
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
dc.subjectcollagen glycosylationen_US
dc.subject.lcshCollagen diseasesen_US
dc.titleLoss of Function of Colgant1 Disrupts Collagen Post-translationa Modification and Causes Musculoskeletal Defectsen_US

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