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P-31 magnetic resonance spectroscopy in skeletal muscle: Experts' consensus recommendations

dc.contributor.authorDamon, Bruce
dc.identifier.citationMeyerspeer M, Boesch C, Cameron D, et al. 31P magnetic resonance spectroscopy in skeletal muscle: Experts' consensus recommendations. NMR in Biomedicine. 2020;e4246.
dc.descriptionOnly Vanderbilt University affiliated authors are listed on VUIR. For a full list of authors, access the version of record at
dc.description.abstractSkeletal muscle phosphorus-31 P-31 MRS is the oldest MRS methodology to be applied to in vivo metabolic research. The technical requirements of P-31 MRS in skeletal muscle depend on the research question, and to assess those questions requires understanding both the relevant muscle physiology, and how P-31 MRS methods can probe it. Here we consider basic signal-acquisition parameters related to radio frequency excitation, TR, TE, spectral resolution, shim and localisation. We make specific recommendations for studies of resting and exercising muscle, including magnetisation transfer, and for data processing. We summarise the metabolic information that can be quantitatively assessed with P-31 MRS, either measured directly or derived by calculations that depend on particular metabolic models, and we give advice on potential problems of interpretation. We give expected values and tolerable ranges for some measured quantities, and minimum requirements for reporting acquisition parameters and experimental results in publications. Reliable examination depends on a reproducible setup, standardised preconditioning of the subject, and careful control of potential difficulties, and we summarise some important considerations and potential confounders. Our recommendations include the quantification and standardisation of contraction intensity, and how best to account for heterogeneous muscle recruitment. We highlight some pitfalls in the assessment of mitochondrial function by analysis of phosphocreatine (PCr) recovery kinetics. Finally, we outline how complementary techniques (near-infrared spectroscopy, arterial spin labelling, BOLD and various other MRI and H-1 MRS measurements) can help in the physiological/metabolic interpretation of P-31 MRS studies by providing information about blood flow and oxygen delivery/utilisation. Our recommendations will assist in achieving the fullest possible reliable picture of muscle physiology and pathophysiology.en_US
dc.description.sponsorshipWe acknowledge contributions to the scientific discussions by David Bendahan, Thomas Jue and Benjamin Chatel. Funded by Austrian Science Fund (FWF) project I 1743-B13 to MM.en_US
dc.publisherNMR in Biomedicineen_US
dc.rights© 2020 The Authors. NMR in Biomedicine published by John Wiley & Sons Ltd This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
dc.subjectnuclear magnetic resonance spectroscopyen_US
dc.subjectphosphorus MRSen_US
dc.titleP-31 magnetic resonance spectroscopy in skeletal muscle: Experts' consensus recommendationsen_US

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