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Microtubule minus-end stability is dictated by the tubulin off-rate

dc.contributor.authorStrothman, Claire
dc.contributor.authorFarmer, Veronica
dc.contributor.authorArpag, Goker
dc.contributor.authorRodgers, Nicole
dc.contributor.authorPodolski, Marija
dc.contributor.authorNorris, Stephen
dc.contributor.authorOhi, Ryoma
dc.contributor.authorZanic, Marija
dc.identifier.citationStrothman, C., Farmer, V., Arpağ, G., Rodgers, N., Podolski, M., Norris, S., Ohi, R., & Zanic, M. (2019). Microtubule minus-end stability is dictated by the tubulin off-rate. The Journal of cell biology, 218(9), 2841–2853.
dc.description.abstractDynamic organization of microtubule minus ends is vital for the formation and maintenance of acentrosomal microtubule arrays. In vitro, both microtubule ends switch between phases of assembly and disassembly, a behavior called dynamic instability. Although minus ends grow slower, their lifetimes are similar to those of plus ends. The mechanisms underlying these distinct dynamics remain unknown. Here, we use an in vitro reconstitution approach to investigate minus-end dynamics. We find that minus-end lifetimes are not defined by the mean size of the protective GTP-tubulin cap. Rather, we conclude that the distinct tubulin off-rate is the primary determinant of the difference between plus- and minus-end dynamics. Further, our results show that the minus-end-directed kinesin-14 HSET/KIFC1 suppresses tubulin off-rate to specifically suppress minus-end catastrophe. HSET maintains its protective minus-end activity even when challenged by a known microtubule depolymerase, kinesin-13 MCAK. Our results provide novel insight into the mechanisms of minus-end dynamics, essential for our understanding of microtubule minus-end regulation in cells.en_US
dc.description.sponsorshipThis work was supported by National Institutes of Health grants R35GM119552 to M. Zanic and R01GM086610 to R. Ohi. C. Strothman acknowledges support from National Institutes of Health grant 5T32GM008554-21. M. Zanic acknowledges support from the Human Frontier Science Program and the Searle Scholars Program. This work was supported in part using the resources of the VIIBRE (Vanderbilt Institute for Integrative Biosystems Research and Education) Microfabrication Core at Vanderbilt University.en_US
dc.publisherJournal of Cell Biologyen_US
dc.rightsCopyright © 2019 Strothman et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at
dc.titleMicrotubule minus-end stability is dictated by the tubulin off-rateen_US

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