Loss of Function of Colgant1 Disrupts Collagen Post-translationa Modification and Causes Musculoskeletal Defects
Geister, Krista A.
Lopez-Jimenez, Alberto Jose
Houghtaling, Scott
Ho, Tzu-Hua
Vanacore, Roberto
Beier, David R.
:
2019-06
Abstract
In a screen for organogenesis defects in N-ethyl-N-nitrosourea (ENU)-induced mutant mice, we discovered a line carrying a mutation in Colgaft1 [collagen beta(1-O)galactosyltransferase type 1], which is required for proper galactosylation of hydroxylysine residues in a number of collagens. Colgaft1 mutant embryos have not been previously characterized; here, we show that they exhibit skeletal and muscular defects. Analysis of mutant-derived embryonic fibroblasts reveals that COLGALT1 acts on collagen IV and VI, and, while collagen VI appears stable and its secretion is not affected, collagen IV accumulates inside of cells and within the extracellular matrix, possibly due to instability and increased degradation. We also generated mutant zebrafish that do not express the duplicated orthologs of mammalian Colgaft1. The double-homozygote mutants have muscle defects; they are viable through the larvae stage but do not survive to 10 days post-fertilization. We hypothesize that the Colgaft1 mutant could serve as a model of a human connective tissue disorder and/or congenital muscular dystrophy or myopathy.