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NMR studies of an α,β-unsaturated aldehyde-derived interstrand carbinolamine DNA cross-link in the 5´-CpG-3´ sequence
(2006-05-01)
The chemistry of acrolein and crotonaldehyde-derived propano-deoxyguanosine (γ-OH-PdG and α-CH<sub>3</sub>-γ-OH-PdG) adducts was monitored in the 5´-CpG-3´ sequence within a dodecamer duplex by NMR spectroscopy, in situ, ...
Biochemical and structural analysis of SV40 Large T antigen: insights into chaperone mediated inactivation of retinoblastoma tumor suppressor protein
(2012-03-28)
Simian Virus 40 uses its Large T antigen protein to bind and inactivate retinoblastoma tumor suppressor proteins, yielding cell transformation. T antigen is a modular protein with four domains connected by linkers. The ...
NMR Resonance Assignments and Secondary Structure of a Mutant Form of the Human KCNE1 Channel Accessory Protein that Exhibits KCNE3-like Function
(2019-03-28)
KCNQ1 (Q1), a voltage-gated potassium channel, is modulated by members of the KCNE family, the best-characterized being KCNE1 (E1) and KCNE3 (E3). The Q1/E1 complex generates a channel with delayed activation and increased ...
Structural and functional analysis of the U-box domain of the E4B ubiquitin ligase
(2011-08-31)
Ubiquitination is a post-translational modification, that functions in a variety of cellular signaling pathways. Unlike modification with a small functional group by a single enzyme, modification by the small protein ...
Conformation of the Aryl Amine DNA Adduct 2-Amino-3-methylimidazole-[4,5-f]quinoline N2-deoxyguanine in the NarI Endonuclease Recognition Sequence as Determined by NMR Spectroscopy
(2015-02-26)
2-Amino-3-methylimidazo-[4,5-f]quinoline, IQ, has been identified as one of the most genotoxic materials according to Ames assays and animal studies. IQ is during high temperature cooking of meats and is classified as ...
Contributions to template priming from the human DNA primase 4Fe-4S cluster domain
(2019-02-12)
Replication of our genes requires unwinding of DNA and generating the new complementary strands for both leading and lagging parental strands. The DNA polymerases that generate the complementary strands require a short ...
Protein structure elucidation by combining computational and experimental methods
(2015-03-25)
Membrane proteins remain a particular challenge in structural biology. Only approximately 1.5% of reported tertiary structures and around 100 unique polytopic membrane proteins are represented in the Protein Data Bank ...
Development and Characterization of NMR and MRI Methods for Assessment of Tumor Oxygen Consumption
(2012-10-08)
A common feature of invasive cancers is that they have an upregulation of glucose metabolism and reduced oxygen utilization. The overall goal of this research was to develop a 17Oxygen MRI method to access this aberrant ...
Oxidative Transformation of Curcumin: Products and Reaction Mechanisms
(2014-04-19)
The dietary polyphenol curcumin shows great promise as an anti-cancer and anti-inflammatory agent. While many cellular targets of curcumin have been identified, the chemical mechanisms whereby these targets are affected ...
Functional Dynamics of Replication Protein A in initiation of SV40 DNA Replication
(2008-04-04)
Human replication protein A (RPA) is a single-stranded DNA (ssDNA) binding protein involved in DNA metabolism. RPA binds ssDNA transiently during initiation of DNA replication. When this dissertation research began, the ...