Radiation-Guided P-selectin Targeted Tumor Imaging in a Lung Tumor Model

Abstract

The objective of this study was to image tumor-targeting using a single chain fragment variable antibody to radiation-induced P-selectin in a heterotopic Lewis lung carcinoma model by near-infrared fluorescence imaging and gamma camera imaging. In our in vitro studies, we observed antibody binding to P-selectin in endothelial cells treated with radiation. In our in vivo optical imaging study, it was observed that significant tumor-specific binding was observed in irradiated tumors expressing P-selectin as compared to unirradiated tumors. Gamma camera imaging also showed successful targeting to P-selectin in irradiated tumors with tumor binding lasting for up to 10 days post-injection. Therefore, radiation-induced P-selectin is a feasible target for the tumor-specific delivery of therapeutic drugs and radionuclides in vivo.