Regulation of RNA Editing by Intracellular Acidification

Abstract

Adenosine deaminases acting on RNA (ADARs) catalyze adenosine-to-inosine (A-to-I) RNA editing to expand the diversity of genomically-encoded transcripts and proteins by hydrolytic deamination of genomically-encoded adenosine residues in metazoan double-stranded RNAs. This RNA processing event is widespread, especially in the human transcriptome, and enables post-transcriptional regulation of many cellular processes. Despite the prevalence and profound biological impact of RNA editing, the mechanisms modulating ADARs and RNA editing are poorly understood. In this work, we compare two different experimental strategies for quantification of A-to-I editing, as well as examine numerous in vitro and in vivo model systems to investigate the modulation of RNA editing.