• About
    • Login
    View Item 
    •   Institutional Repository Home
    • Electronic Theses and Dissertations
    • Electronic Theses and Dissertations
    • View Item
    •   Institutional Repository Home
    • Electronic Theses and Dissertations
    • Electronic Theses and Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of Institutional RepositoryCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsDepartmentThis CollectionBy Issue DateAuthorsTitlesSubjectsDepartment

    My Account

    LoginRegister

    MECHANISTIC STUDIES OF REPLICATION FORK REMODELING BY DNA TRANSLOCASES

    Warren, Garrett M
    : https://etd.library.vanderbilt.edu/etd-05152019-104144
    http://hdl.handle.net/1803/15420
    : 2019-05-15

    Abstract

    During DNA replication the replication fork can become stalled by impediments that prevent complete and accurate duplication of the genome. DNA replication fork reversal is an important pathway for the protection and restoration of stalled DNA replication forks in both prokaryotes and eukaryotes to preserve genome stability. Several enzymes known as fork remodelers initiate this pathway by actively reversing a stalled DNA replication fork, although their mechanisms of action are not well understood. This dissertation presents work characterizing fork reversal mechanisms of the prokaryotic fork remodeler RecG and the eukaryotic SNF2-family fork remodelers SMARCAL1, ZRANB3, and HLTF. Biophysical analysis of RecG binding to a model DNA replication fork revealed how conformational changes within the motor domain upon binding DNA is required for efficient fork reversal activity. Comparison of fork reversal mechanisms of the eukaryotic fork remodelers show differences in sensitivity to DNA damage on the leading and lagging strand template, providing a rationale for the multiple non-redundant fork remodeling activities in the cell. This work has expanded our knowledge of how these enzymes recognize and remodel stalled replication forks to ensure genome stability.
    Show full item record

    Files in this item

    Icon
    Name:
    Warren_Dissertation.pdf
    Size:
    4.511Mb
    Format:
    PDF
    View/Open

    This item appears in the following collection(s):

    • Electronic Theses and Dissertations

    Connect with Vanderbilt Libraries

    Your Vanderbilt

    • Alumni
    • Current Students
    • Faculty & Staff
    • International Students
    • Media
    • Parents & Family
    • Prospective Students
    • Researchers
    • Sports Fans
    • Visitors & Neighbors

    Support the Jean and Alexander Heard Libraries

    Support the Library...Give Now

    Gifts to the Libraries support the learning and research needs of the entire Vanderbilt community. Learn more about giving to the Libraries.

    Become a Friend of the Libraries

    Quick Links

    • Hours
    • About
    • Employment
    • Staff Directory
    • Accessibility Services
    • Contact
    • Vanderbilt Home
    • Privacy Policy