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A transcription factor network dictates neuronal cell fate decisions in the zebrafish dorsal diencephalon

dc.creatorKhuansuwan, Sataree
dc.date.accessioned2020-08-22T21:06:53Z
dc.date.available2016-09-25
dc.date.issued2014-09-25
dc.identifier.urihttps://etd.library.vanderbilt.edu/etd-09242014-175729
dc.identifier.urihttp://hdl.handle.net/1803/14223
dc.description.abstractThe zebrafish epithalamus, which includes the dorsal habenular nuclei and pineal complex, displays robust molecular and anatomical asymmetries. The full elaboration of molecular and anatomical differences between the left and right dorsal habenular nuclei depends on the formation of a left-sided parapineal organ. This work has yielded significant insights into the role of Tbx2b during parapineal development using both the candidate gene approach as well as the non-biased, transcriptome analysis approach. Through the candidate approach, it was determined that Tbx2b acts in a permissive manner during parapineal specification, but it is necessary for proper parapineal migration. Further, the inhibitory roles of Flh and Nr2e3 during formation of parapineal neurons have been characterized. Using RNA-sequencing to perform differential expression analysis between wild-type and tbx2b-deficient pineal complex cells has yielded many targets that are downstream of Tbx2b. Together, these studies also provided a novel insight into a potential genetic interaction system between Tbx2b and Nr2e3 during cone versus rod photoreceptor differentiation.
dc.format.mimetypeapplication/pdf
dc.subjectparapineal
dc.subjectleft-right asymmetry
dc.subjectDanio rerio
dc.subjectzebrafish
dc.subjectTbx2b
dc.subjectpineal
dc.subjectpineal complex
dc.subjecttranscriptome analysis
dc.subjectFlh
dc.subjectNr2e3
dc.subjectFACS
dc.titleA transcription factor network dictates neuronal cell fate decisions in the zebrafish dorsal diencephalon
dc.typedissertation
dc.contributor.committeeMemberWenbiao Chen
dc.contributor.committeeMemberJoshua T. Gamse
dc.contributor.committeeMemberDouglas G. McMahon
dc.contributor.committeeMemberKevin Ess
dc.type.materialtext
thesis.degree.namePHD
thesis.degree.leveldissertation
thesis.degree.disciplineBiological Sciences
thesis.degree.grantorVanderbilt University
local.embargo.terms2016-09-25
local.embargo.lift2016-09-25
dc.contributor.committeeChairJames G. Patton


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