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The role of antimicrobial skin peptides in defense of leopard frogs against chytridiomycosis

dc.creatorPask, James David
dc.date.accessioned2020-08-22T00:03:09Z
dc.date.available2013-04-15
dc.date.issued2013-04-15
dc.identifier.urihttps://etd.library.vanderbilt.edu/etd-03262013-101535
dc.identifier.urihttp://hdl.handle.net/1803/11430
dc.description.abstractGlobal amphibian populations, including the northern leopard frog (Rana pipiens), have been declining for the past four decades, and overwhelming evidence now links a pathogenic chytrid fungus, Batrachochytrium dendrobatidis (Bd) to declines across six continents. Because Bd colonizes the skin, antimicrobial peptides secreted onto the surface of the skin are thought to be an important component of the immune defenses against this pathogen. My studies showed that resting frogs constitutively secrete low levels of AMPs that increase following a natural activity such as movement to escape a predator. Using MALDI-TOF MS and growth inhibition assays, I showed that AMPs are present on the skin for at least two hours but are degraded in a time-dependent fashion. Injection of 40 nmol/g norepinephrine results in a long-term depletion of skin peptides. When skin peptides were depleted in juvenile frogs by norepinephrine injection, the frogs were more susceptible to Bd infection and died more rapidly than control frogs with an intact set of skin peptides. These studies support the hypothesis that AMPs are an important defense for this species against chytridiomycosis.
dc.format.mimetypeapplication/pdf
dc.subjectantimicrobial peptides
dc.titleThe role of antimicrobial skin peptides in defense of leopard frogs against chytridiomycosis
dc.typedissertation
dc.contributor.committeeMemberKevin Schey
dc.contributor.committeeMemberLouise Rollins-Smith
dc.contributor.committeeMemberTom Aune
dc.contributor.committeeMemberJohn Williams
dc.type.materialtext
thesis.degree.namePHD
thesis.degree.leveldissertation
thesis.degree.disciplineMicrobiology and Immunology
thesis.degree.grantorVanderbilt University
local.embargo.terms2013-04-15
local.embargo.lift2013-04-15
dc.contributor.committeeChairLuc Van Kaer


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