Show simple item record

The role of the murine EP3 receptor variants on cell function.

dc.creatorMacias-Perez, Ines Maria
dc.description.abstractProstaglandin E2 (PGE2), which exerts its functions by binding to four G protein-coupled receptors (EP1-4), is implicated in tumorigenesis. Among the four EP receptors, EP3 is unique in that it exists as alternatively spliced variants, characterized by differences in the cytoplasmic C-terminal tail. Although three EP3 variants á, â and ã have been described in mice, their functional significance in regulating tumorigenesis is unknown. In this study we provide evidence that expressing murine EP3 á, â and ã receptor variants in tumor cells reduces to the same degree their tumorigenic potential in vivo. In addition, activation of each of the three mEP3 variants induces enhanced cell-cell contact and reduces cell proliferation in vitro in a Rho-dependent manner. Finally, we demonstrate that EP3- mediated RhoA activation requires the engagement of the heterotrimeric G protein G12. Thus, our study provides strong evidence that selective activation of each of the three variants of the EP3 receptor suppresses tumor cell function by activating a G12-RhoA pathway.
dc.subjectEP receptor
dc.subjectProstaglandins -- Receptors
dc.subjectCyclooxygenase 2
dc.subjectColon (Anatomy) -- Cancer -- Molecular aspects
dc.titleThe role of the murine EP3 receptor variants on cell function.
dc.contributor.committeeMemberRichard Breyer
dc.contributor.committeeMemberRichard Peek
dc.contributor.committeeMemberLynn Matrisian
dc.type.materialtext Biology University
dc.contributor.committeeChairAmbra Pozzi

Files in this item


This item appears in the following Collection(s)

Show simple item record